Analysis of Environmental Humidity on DNA Degredation

Abstract

DARIANA ADKINS and MARK R. FLOOD, Forensic Science Program, Fairmont State University, Fairmont, WV, 26654. Analysis of Environmental Humidity on DNA Degradation.

There are a multitude of DNA samples sitting in labs, degrading by the minute. Temperature, humidity, and containment are the largest factors in speeding up the process of DNA degradation. This study aims to analyze the effects of different environmental humidity conditions on DNA sample degradation.  DNA was extracted from buccal swabs using a Bio-Rad Instagene Matrix protocol.  Fresh samples and samples stored at room temperature for various time periods, with and without desiccation, were compared.  The preliminary data shows that fresh samples have more DNA extracted than stored samples, and the desiccation with a water absorbing material (DRIERITE) may increase the concentration of DNA extracted.  Future goals of this project include the amplification of the DNA extracted to assess the DNA quality. Our preliminary data shows that keeping collected DNA samples in a low humidity environment may increase the retention of DNA compared to the samples sitting in open air.

This research was made possible by NASA West Virginia Space Grant Consortium, Training Grant #80NSSC20M0055.