Effects of Nicotine on Chicken Embryonic Development
DOI:
https://doi.org/10.55632/pwvas.v89i2.149Keywords:
Nicotine, Chicken, Neural DevelopmentAbstract
A large proportion of the United States population has been exposed to maternal smoking in utero. Mounting data suggests that nicotine can have a negative impact on neural system development. The goal of this study was to evaluate effects of nicotine exposure on chicken neural system. The early chick embryo is an established model of the first month of embryonic development in mammals. Nicotine (nicotine hydrogen bitartrate) or vehicle (sodium bitartrate monohydrate) solutions were injected in eggs prior to incubation (300ng/ml). Three cohorts of 24 eggs distributed between treatment groups were generated. After injections, eggs were sealed and placed in the incubator. Embryos were harvested on day 5 after injections, evaluated and measured, embedded in paraplast, sectioned, and stained with hematoxylin and eosin for histological analysis. Our data indicates that the nicotine treatment does not affect viability, weight, or length of the embryos. Nonetheless, nicotine notably affects the axial rotation of the embryos (defined as a change in the dorsoventral orientation of the head during development). In this study, altered axial rotation was observed in nicotine treated groups 4 times more often than in controls (p<0.05). Microscopic analysis demonstrated that atypical axial rotation was associated with incomplete closing of the embryonic neural tube in the cervical region, but not in other areas of the tube. Further research is needed to evaluate the exact mechanisms of the developmental insult onto neural system development observed in the present study.References
Abou-Donia, M. B., W. A. Khan, et al. (2006). "In utero exposure to nicotine and chlorpyrifos alone, and in combination produces persistent sensorimotor deficits and Purkinje neuron loss in the cerebellum of adult offspring rats." Arch Toxicol 80(9): 620-31.
Atluri, P., M. W. Fleck, et al. (2001). "Functional nicotinic acetylcholine receptor expression in stem and progenitor cells of the early embryonic mouse cerebral cortex." Dev Biol 240(1): 143-56.
Berger, F., F. H. Gage, et al. (1998). "Nicotinic receptor-induced apoptotic cell death of hippocampal progenitor cells." J Neurosci 18(17): 6871-81.
Bohn, N., V. Humphrey, et al. (2014). Effects of Nicotine on Chicken Embryonic Development.
West Virginia Academy of Science 89th Annual Meeting
Shepherd University, Shepherdstown, WV: 54.
Buznikov, G. A., Y. B. Shmukler, et al. (1996). "From oocyte to neuron: do neurotransmitters function in the same way throughout development?" Cell Mol Neurobiol 16(5): 537-59.
Centers for Disease, C. and Prevention (2010). "State-specific prevalence of cigarette smoking and smokeless tobacco use among adults --- United States, 2009." MMWR Morb Mortal Wkly Rep 59(43): 1400-6.
Chen, W. J., S. E. Parnell, et al. (1999). "Effects of alcohol and nicotine on developing olfactory bulb: loss of mitral cells and alterations in neurotransmitter levels." Alcohol Clin Exp Res 23(1): 18-25.
Chiolero, A., P. Bovet, et al. (2005). "Association between maternal smoking and low birth weight in Switzerland: the EDEN study." Swiss Med Wkly 135(35-36): 525-30.
Dalgic, A., E. Armagan, et al. (2009). "High dose cotinine may induce neural tube defects in a chick embryo model." Turk Neurosurg 19(3): 224-9.
Greenberg, M. E., E. B. Ziff, et al. (1986). "Stimulation of neuronal acetylcholine receptors induces rapid gene transcription." Science 234(4772): 80-3.
Hamburger, V. and H. L. Hamilton (1951). "A series of normal stages in the development of the chick embryo." J Morphol 88(1): 49-92.
Hohmann, C. F. (2003). "A morphogenetic role for acetylcholine in mouse cerebral neocortex." Neurosci Biobehav Rev 27(4): 351-63.
Humprey, V., N. Bohn, et al. (2014). A single nicotine injection leads to neural tube defects in chicken model. Society for Neuroscience Annual Meeting. Washington, DC: 619.18
Joschko, M. A., I. E. Dreosti, et al. (1991). "The teratogenic effects of nicotine in vitro in rats: a light and electron microscope study." Neurotoxicol Teratol 13(3): 307-16.
Li, Z., L. Zhang, et al. (2012). "Partner cigarette smoking and risk of neural tube defects among infants of non-smoking women in northern China." Tob Control 22(6): 401-5.
Lichtensteiger, W., U. Ribary, et al. (1988). "Prenatal adverse effects of nicotine on the developing brain." Prog Brain Res 73: 137-57.
Malcoe, L. H., G. M. Shaw, et al. (1999). "The effect of congenital anomalies on mortality risk in white and black infants." Am J Public Health 89(6): 887-92.
Manca, A., S. Capsoni, et al. (2012). "Nerve growth factor regulates axial rotation during early stages of chick embryo development." Proc Natl Acad Sci U S A 109(6): 2009-14.
Navarro, H. A., F. J. Seidler, et al. (1989). "Prenatal exposure to nicotine impairs nervous system development at a dose which does not affect viability or growth." Brain Res Bull 23(3): 187-92.
Nguyen, L., J. M. Rigo, et al. (2001). "Neurotransmitters as early signals for central nervous system development." Cell Tissue Res 305(2): 187-202.
Onal, A., A. Uysal, et al. (2004). "Alterations of brain tissue in fetal rats exposed to nicotine in utero: possible involvement of nitric oxide and catecholamines." Neurotoxicol Teratol 26(1): 103-12.
Roebroek, A. J., L. Umans, et al. (1998). "Failure of ventral closure and axial rotation in embryos lacking the proprotein convertase Furin." Development 125(24): 4863-76.
Roy, T. S., J. E. Andrews, et al. (1998). "Nicotine evokes cell death in embryonic rat brain during neurulation." J Pharmacol Exp Ther 287(3): 1136-44.
Roy, T. S. and U. Sabherwal (1994). "Effects of prenatal nicotine exposure on the morphogenesis of somatosensory cortex." Neurotoxicol Teratol 16(4): 411-21.
Roy, T. S. and U. Sabherwal (1998). "Effects of gestational nicotine exposure on hippocampal morphology." Neurotoxicol Teratol 20(4): 465-73.
Roy, T. S., F. J. Seidler, et al. (2002). "Prenatal nicotine exposure evokes alterations of cell structure in hippocampus and somatosensory cortex." J Pharmacol Exp Ther 300(1): 124-33.
Schneider, A. S., P. Atluri, et al. (2002). "Functional nicotinic acetylcholine receptor expression on stem and progenitor cells of the early embryonic nervous system." Ann N Y Acad Sci 971: 135-8.
Seidler, F. J., E. S. Albright, et al. (1994). "In search of a mechanism for receptor-mediated neurobehavioral teratogenesis by nicotine: catecholamine release by nicotine in immature rat brain regions." Brain Res Dev Brain Res 82(1-2): 1-8.
Slotkin, T. A., E. C. McCook, et al. (1997). "Cryptic brain cell injury caused by fetal nicotine exposure is associated with persistent elevations of c-fos protooncogene expression." Brain Res 750(1-2): 180-8.
Smith, S. J. (1994). "Neurobiology. Just a chemical attraction." Nature 368(6467): 101-2.
Trauth, J. A., F. J. Seidler, et al. (1999). "Persistent c-fos induction by nicotine in developing rat brain regions: interaction with hypoxia." Pediatr Res 45(1): 38-45.
Tribollet, E., D. Bertrand, et al. (2004). "Comparative distribution of nicotinic receptor subtypes during development, adulthood and aging: an autoradiographic study in the rat brain." Neuroscience 124(2): 405-20.
Wang, M., Z. P. Wang, et al. (2014). "Maternal smoking during pregnancy and neural tube defects in offspring: a meta-analysis." Childs Nerv Syst 30(1): 83-9.
Yi, Y., M. Lindemann, et al. (2011). "Economic burden of neural tube defects and impact of prevention with folic acid: a literature review." Eur J Pediatr 170(11): 1391-400.
Zoli, M., N. Le Novere, et al. (1995). "Developmental regulation of nicotinic ACh receptor subunit mRNAs in the rat central and peripheral nervous systems." J Neurosci 15(3 Pt 1): 1912-39.
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