Differential Gene Expression of Prdm14 That Could Contribute to Sexual Dimorphisms

Authors

  • Shelby Shajimon Shepherd University
  • Jackie Huhn Temple University
  • Rachel Werner Temple University
  • Robert Warburton Shepherd University
  • Nora Engel Temple University

Abstract

Sexual dimorphisms are apparent in differences in disease susceptibilities, types, onset and response to therapy. Previous studies in mouse embryonic stem (ES) cells as models of early embryogenesis showed that there were many genes that were differentially expressed in male and female cells. One of these genes codes for Prdm14, a gene involved in the pluripotency state of embryonic stem cells. This study explores the regulatory differences that lead to higher expression of Prdm14 in female ES cells with a dual luciferase reporter assay. The enhancer of Prdm14 harbors motifs responsive to Prdm14 itself, thus establishing an auto-regulatory loop. These motifs were studied, either by deletion or scrambling of the sequence using site-directed mutagenesis. Our data shows that there are significant differences in expression of Prdm14 in XX ESC when compared to XY and XO lines. Thus, it can be concluded that the activity of the Prdm14 enhancer is dosage-sensitive. We also report on experiments designed to detect methylation differences in  XX, XY, and XO ES cell lines.

Author Biographies

Shelby Shajimon, Shepherd University

Department of Biology

Jackie Huhn, Temple University

The Fels Institute For Cancer Research and Molecular Biology

Rachel Werner, Temple University

The Fels Institute For Cancer Research and Molecular Biology

Robert Warburton, Shepherd University

Professor of Biochemistry

Department of Chemistry

Nora Engel, Temple University

The Fels Institute For Cancer Research and Molecular Biology

Associate Professor, Medical Genetics and Molecular Biochemistry

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Published

2017-04-04

How to Cite

Shajimon, S., Huhn, J., Werner, R., Warburton, R., & Engel, N. (2017). Differential Gene Expression of Prdm14 That Could Contribute to Sexual Dimorphisms. Proceedings of the West Virginia Academy of Science, 89(1). Retrieved from https://pwvas.org/index.php/pwvas/article/view/240

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Section

Meeting Abstracts-Poster