Lyme disease and tick (Acari: Ixodidae) monitoring in Upshur County, WV

Authors

  • Mason N Benchoff West Virginia Wesleyan College
  • Lukas Z Samargo West Virginia Wesleyan College
  • Melanie S Sal West Virginia Wesleyan College
  • Kim Bjorgo-Thorne West Virginia Wesleyan College

Keywords:

Borrelia, Ixodes, monitoring,

Abstract

Lyme disease is one of the most widespread vector-borne diseases in the Northern Hemisphere. In North America, it is mainly caused by the spirochete Borrelia burgdorferi. The main known transmission vector for B. burgdorferi is Ixodes scapularis, commonly referred to as the black legged tick. Through conducting this experiment, the presence and viability of Borrelia burgdorferi found within Ixodes scapularis and the prevalence of tick species found within West Virginia were examined.  We collected different species of ticks via drag method in various locations throughout Upshur County,West Virginia. These ticks were identified, had their DNA extracted and amplified via PCR, and were run through gel electrophoresis to detect presence of B. burgdorferi. Results showed that around 5% of ticks collected contained the bacterium B. burgdorferi and a handful of other ticks showed non-specific bands of unknown extraneous DNA. Future studies include looking into a new, invasive tick recently introduced to the area and its capability to carry B. burgdorferi, as well as acquiring Rickettsia DNA and using a positive control containing it, to examine if the unidentified region makers found in the gels coincide with that particular bacterium

Author Biographies

Mason N Benchoff, West Virginia Wesleyan College

Student

Lukas Z Samargo, West Virginia Wesleyan College

Student

Melanie S Sal, West Virginia Wesleyan College

Associate Professor

Chair, Department of Biology & Environmental Science

Kim Bjorgo-Thorne, West Virginia Wesleyan College

Associate professor

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Published

2019-03-20

How to Cite

Benchoff, M. N., Samargo, L. Z., Sal, M. S., & Bjorgo-Thorne, K. (2019). Lyme disease and tick (Acari: Ixodidae) monitoring in Upshur County, WV. Proceedings of the West Virginia Academy of Science, 91(1). Retrieved from https://pwvas.org/index.php/pwvas/article/view/601

Issue

Section

Meeting Abstracts-Poster