The effect of the antibiotic Spectramast LC on the growth of E. coli in the rumen of dairy cattle.
DOI:
https://doi.org/10.55632/pwvas.v92i1.655Keywords:
Antibiotic, Dairy cattle, Rumen, E. coliAbstract
Many microorganisms call the gastrointestinal tract of a cow home. With the growing use of antibiotics in the field of agriculture, it is very important to understand the effects of antibiotics on the microorganisms in the rumen. In this study we examined the effect of the antibiotic Spectramast LC on the indicator bacteria species E. coli. Fresh fecal samples were obtained from Holstein cattle on and off Spectramast LC. Samples were then diluted and placed on Mueller Hinton plates. Blank antibiotic disks were in Spectramast LC and placed in quadrants on the plate, and after 24 and 48 hours the zones of inhibition were measured. Preliminary results indicate a significant effect of Spectramast LC on the growth of E. coli. Implications for cattle health will be discussed.
References
Abera, B., Kibret, M. 2011. Antimicrobial susceptibility patterns of E. coli from clinical sources in northeast Ethiopia. African Health Sciences. 11(S1): S40 - S45
Auffret, M.D., et al. 2017. The rumen microbiome as a reservoir of antimicrobial resistance and pathogenicity genes is directly affected by diet in beef cattle. Microbiome. 5(159): DOI 10.1186/s40168-017-0378-z
Diez-Gonzalez, et al. 1998. Grain Feeding and the Dissemination of Acid-Resistant Escherichia coli from Cattle. American Association for the Advancement of Science. 281(5383): 1666-1668
Fuenzalida, M.J., Ruegg, P.L. 2019. Negatively controlled, randomized clinical trial to evaluate use of intramammary ceftiofur for treatment of nonsevere culture-negative clinical mastitis. 102(4): 3321-3338
Gordon, P.J. 2017. Impact of disease in dairy cows on ceftiofur pharmacokinetics, withdrawal times and emergence of antimicrobial resistance. Iowa State University. 1-178
Heider, L.C. 2011. Studies on Salmonella enterica and Escherichia coli with a focus on ceftiofur and the genetic resistance determinant blaCMY-2. The Ohio State University. 1-120
Ji, S., et al. 2018. Ecological Restoration of Antibiotic-Disturbed Gastrointestinal Microbiota in Foregut and Hindgut of Cows. Frontiers in Cellular and Infection Microbiology. 8(79). DOI: 10.3389/fcimb.2018.00079
Maki, L.R., Picard, K. 1964. Normal Intestinal Flora of Cattle Fed High-Roughage Rations. Journal of Bacteriology. 89(5): 1244-1249
Truchetti, G., et al. 2014.
Efficacy of extended intramammary ceftiofur therapy against mild to moderate clinical mastitis in Holstein dairy cows: A randomized clinical trial. The Canadian Journal of Veterinary Research. 78:31–37
Vasquez, A.M. 2018. Prudent use of Antimicrobials on Dairy Farms: Alternative Protocols for the Treatment or Prevention of Mastitis and Associated Outcomes. Cornell University. 1-227
Wichmann, F., et al. 2014. Diverse Antibiotic Resistance Genes in Dairy Cow Manure. mBio. 5(2): 1-9
Downloads
Published
How to Cite
Issue
Section
License
Proceedings of the West Virginia Academy of Science applies the Creative Commons Attribution-NonCommercial (CC BY-NC) license to works we publish. By virtue of their appearance in this open access journal, articles are free to use, with proper attribution, in educational and other non-commercial settings.