Optimizing a micro-fluidic slide for in vivo imaging of larval Zebrafish.

Authors

  • Dakota James Parnell Bluefield State College
  • Jesse Reed Orell Bluefield State College
  • Syed Monis Ali Bluefield State College
  • James William Walters Bluefield State College

Keywords:

micro-fluidics, Zeiss, mounting

Abstract

Larval zebrafish (Danio rerio) are optically transparent at six- and seven-days post fertilization (dpf) which makes them useful research models for the study of metabolic and physiological processes. Previous methods to image zebrafish were limited to embryogenesis and early larval stages. Our goal was to design a novel micro-fluidic slide for mounting zebrafish that greatly extends the duration of time for in vivo imaging of zebrafish larvae that are six to seven days post fertilization (dpf). The ideal mounting slide will have channels to provide media exchange, nourishment, and/or drug treatments and channels to remove waste and flush the system of previous test conditions. We had a microfluidic slide manufactured to our specifications. Initial testing revealed that the larval bed was too large. To optimize our previous prototype, we used the Zeiss Discovery V8 microscope to recalculate our measurements for the larvae bed. We have now re-imaged five each of six dpf larvae and five each of seven dpf larvae in order to adjust the measurements to the new average larval size. This work was supported by NIH Grant P20GM103434 to the West Virginia IDeA Network for Biomedical Research Excellence and NIH Grant P20GM103434 awarded to Bluefield State College

Author Biographies

Dakota James Parnell, Bluefield State College

Mcnair Scholar and Student Researcher

Jesse Reed Orell, Bluefield State College

Student Researcher, McNair Scholar

Syed Monis Ali, Bluefield State College

McNair Scholar

James William Walters, Bluefield State College

Chair of Applied Science and Mathematics, Associate professor of biology.

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Published

2020-04-29

How to Cite

Parnell, D. J., Orell, J. R., Ali, S. M., & Walters, J. W. (2020). Optimizing a micro-fluidic slide for in vivo imaging of larval Zebrafish. Proceedings of the West Virginia Academy of Science, 92(1). Retrieved from https://pwvas.org/index.php/pwvas/article/view/736

Issue

Section

Meeting Abstracts-Poster